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. 2010 Mar;129(3):437–445. doi: 10.1111/j.1365-2567.2009.03199.x

Figure 1.

Figure 1

Uptake of ovalbumin (OVA) and advanced glycation endproduct (AGE)-OVA by immature dendritic cells (DCs) with or without inhibitors. (a) Immature DCs were loaded with 10 μg/ml fluorescein isothiocyanate (FITC)-conjugated OVA or AGE-OVA and their uptake was detected by flow cytometry after 10, 60 and 240 min. The inhibitors mannan (200 μg/ml), poly I (20 μg/ml), and dimethylamiloride (DMA) (300 μm) were added to the cells 30 min before the addition of the allergens. The mean ± standard deviation of eight experiments is shown. *P ≤ 0·05 compared with OVA; §P ≤ 0·01 compared with no inhibition. (b) One representative experiment of the uptake of FITC-conjugated OVA and AGE-OVA after 240 min with inhibitors. (c) Immature DCs were treated with goat anti-human receptor for advanced glycation endproducts (RAGE) antibody (1 μg/ml) 30 min prior to application of the allergens and analysed by fluorescence microscopy after 4 hr.