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. Author manuscript; available in PMC: 2010 Feb 23.
Published in final edited form as: Br J Nutr. 2009 Aug;102(4):497–501. doi: 10.1017/S0007114509231758

Table 1.

Fatty acid composition (mol %) and cholesterol content (mg/100 mg protein) of macrophages differentiated from THP-1 cells* (Mean values and standard deviations)

Control
MA
PA
LA
AA
EPA
DHA
Fatty acid Mean sd Mean sd Mean sd Mean sd Mean sd Mean sd Mean sd
SFA 46.08b,c 3.35 59.64a 1.16 54.46a,b 1.73 31.95e 0.79 36.23d,e 0.87 36.17d,e 1.31 42.60d 7.44
 12 : 0 0.42b,c 0.07 0.54a,b 0.08 0.31c 0.06 0.23c 0.04 0.31c 0.03 0.28c 0.02 0.61a 0.12
 14 : 0 3.58b,c 0.51 20.35a 0.37 3.10b,c 0.36 2.67c 0.18 3.42b,c 0.23 3.53b,c 0.31 4.15b 0.51
 16 : 0 29.26b 2.25 29.05b 0.14 39.48a 1.76 21.01c 0.51 24.29b,c 0.73 24.73b,c 1.08 28.75b 5.09
 18 : 0 11.17a 1.35 8.10b,c 0.53 10.42a,b 0.44 7.26c 0.06 7.49c 0.06 6.80c 0.19 8.08b,c 1.77
 20 : 0 0.33a 0.03 0.25b 0.02 0.35a 0.03 0.16c 0.01 0.17c 0.01 0.16c 0.00 0.17c 0.04
 22 : 0 0.28a 0.04 0.17b,c,d 0.02 0.22a,b 0.02 0.10e 0.02 0.13c,d,e 0.02 0.19b,c 0.02 0.12d,e 0.02
 24 : 0 1.03a 0.15 0.58b,c 0.04 0.57b,c 0.08 0.45c 0.06 0.42c 0.06 0.49b,c 0.02 0.73b 0.17
MUFA 39.78a 2.75 30.13b 2.24 34.31b 1.31 20.54c 2.06 21.18c 1.70 20.47c 1.29 20.59c 1.91
 16 : 1n-9 1.02a 0.17 0.76a,b 0.10 0.81a,b 0.10 0.66b 0.03 0.76a,b 0.11 0.77a,b 0.04 0.71b 0.06
 16 : 1n-7 4.54a,b 1.20 4.45a,b 0.83 4.72a 0.67 1.89c 0.26 2.62b,c 0.58 2.81a,b,c 0.42 2.73b,c 0.43
 18 : 1n-9 22.99a 1.09 16.23c 0.79 19.02b 0.15 11.62d 0.93 11.96d 0.56 11.53d 0.40 11.86d 1.17
 18 : 1n-7 10.07a 1.02 7.99b 0.88 8.69a,b 0.68 5.59c 0.65 5.18c 0.36 4.60c 0.18 4.83c 0.37
 20 : 1n-9 0.70a 0.08 0.44b 0.04 0.65a 0.11 0.38b,c 0.03 0.24c,d 0.04 0.18d 0.04 0.20d 0.03
 24 : 1n-9 0.46 0.33 0.26 0.07 0.42 0.20 0.39 0.24 0.42 0.34 0.57 0.46 0.26 0.23
PUFA 14.14b 3.94 10.23b 1.65 11.24b 2.63 47.51a 2.80 42.59a 2.56 43.35a 2.55 36.80a 8.74
n-6 PUFA 11.36b 2.97 8.18b 1.34 8.86b 2.05 45.13a 2.51 40.57a 2.13 8.28b 1.13 8.25b 1.84
 18 : 2 2.73b 0.44 1.91b 0.24 1.95b 0.20 36.79a 2.17 2.12b 0.24 1.96b 0.09 1.91b 0.15
 18 : 3 0.16 0.14 0.22 0.17 0.24 0.04 0.23 0.13 0.39 0.13 0.43 0.12 0.20 0.05
 20 : 3 1.59b 0.47 1.09b 0.22 1.38b 0.62 2.00a,b 0.02 3.55a 0.98 1.20b 0.29 1.64b 1.00
 20 : 4 5.99b 1.64 4.33b 0.65 4.51b 1.01 4.60b 0.34 24.39a 1.33 3.88b 0.46 4.11b 0.84
 22 : 2 0.30 0.19 0.22 0.07 0.34 0.28 0.53 0.14 0.26 0.28 0.21 0.23 0.03 0.02
 22 : 4 0.50c 0.11 0.37c 0.01 0.41c 0.05 0.89b 0.07 9.49a 0.30 0.54b,c 0.03 0.32c 0.02
 22 : 5 0.09b 0.08 0.03b 0.02 0.03b 0.01 0.07b 0.05 0.37a 0.10 0.05b 0.03 0.04b 0.03
n-3 PUFA 2.79b 0.98 2.05b 0.31 2.38b 0.58 2.39b 0.97 2.02b 0.47 35.08a 1.44 28.55a 7.68
 18 : 3 0.23a,b 0.02 0.15b 0.01 0.14b 0.04 0.15b 0.01 0.14b 0.04 0.33a 0.09 0.16b 0.06
 20 : 5 0.80b,c 0.36 0.59c 0.19 0.84b,c 0.43 0.86b,c 0.96 0.38c 0.14 18.75a 1.43 3.34b 1.85
 22 : 5 0.51c 0.16 0.40c 0.01 0.43c 0.08 0.61b,c 0.11 0.65b,c 0.07 14.92a 0.34 1.07b 0.17
 22 : 6 1.24b 0.50 0.91b 0.11 0.98b 0.11 0.77b 0.20 0.85b 0.22 1.07b 0.31 23.98a 6.36
TC 1.36 0.19 1.41 0.32 1.43 0.34 1.39 0.03 1.42 0.10 1.50 0.07 1.21 0.07
FC 1.29 0.19 1.22 0.34 1.25 0.31 1.24 0.00 1.16 0.10 1.20 0.05 1.03 0.06
EC 0.07c 0.01 0.19b 0.04 0.18b 0.03 0.15b 0.03 0.27a 0.01 0.30a 0.02 0.18b 0.01

MA, myristic acid; PA, palmitic acid; LA, linoleic acid; AA, arachidonic acid; TC, total cholesterol; FC, non-esterified cholesterol; EC, esterified cholesterol.

a–e

Mean values within a row with unlike superscript letters were significantly different (P<0.05).

*

Each experiment was performed in three independent cell cultures.