Figure 1.

A: Light microscopy of human prenatal cortical progenitor cells (hNPC^ctx) cultured as neurospheres; B: fluorescence microscopy of GFP transduced hNPC^ctx; C: Visual thresholds from native hNPC^ctx, prelabeled hNPC^ctx-GFP, sham and untreated eyes from RCS rats (n=10/group) tested at four time points. There is no difference between native hNPC^ctx and hNPC^ctx-GFP treated groups (p>0.05); however, significantly different between cell-injected and shame/untreated controls (p<0.01). Two weeks after subretinal injection of hNPC^ctx, the distribution of donor cells (arrowed) is similar, as shown in D (retinal cross section with donor cells stained with human nuclear marker on cresyl violet background) and E, (hNPC^{ctx –}GFP shown green). No donor cells were seen in the sensory retina at any time.