Skip to main content
. Author manuscript; available in PMC: 2011 Feb 19.
Published in final edited form as: Circ Res. 2010 Feb 19;106(3):479–494. doi: 10.1161/CIRCRESAHA.109.208991

Figure 4.

Figure 4

A: Standard curve correlating ex vivo luciferase activity in rat heart homogenates with known cell numbers of luciferase-expressing cardiac derived stem cells, indicating the excellent sensitivity and linearity of the assay. B: Absolute quantification of CDC number at 24hrs after direct intramyocardial injection, in 3 rats, using ex vivo luciferase assay. C: A quantitative real time PCR standard curve used for ex vivo quantification of male cell numbers injected in female hearts. A genomic sequence of the SRY gene is used as target. The curve shows excellent sensitivity of the assay, high efficiency of the reaction and linearity of the relationship between SRY gene copies and cell numbers. D: amplification plots of the reactions corresponding to the points of the standard curve. Data were analyzed with the SDS 2.1 software, by Applied Biosystems.