Figure 4.

NT5E protein and AMP hydrolytic activity are reduced in axon terminals following peripheral nerve injury. A–D, Sections of lumbar spinal cord from a mouse killed 14 d after ligation and transection of the sural and common peroneal branches of the sciatic nerve (the SNI model). A, Stained using AMP histochemistry (3 mm AMP; buffer pH was 7.0). B–D, An adjacent section labeled for the indicated markers. Arrowheads delimit the region of dorsal spinal cord where staining was reduced ipsilateral (ipsi) to the injured nerves. Contralateral (contra), uninjured side. Similar results were seen in n = 2 mice. Scale bar: (in D) A–D, 200 μm.