Figure 5.

AMP hydrolytic activity is reduced in nociceptive circuits of Nt5e^−/− mice. A–H, Lumbar DRG (A, B), lumbar spinal cord (C–F) and cultured DRG neurons (G, H) from WT and Nt5e^−/− adult mice stained using AMP histochemistry. Arrows point to epineurium (en). C, D, Arrowheads mark the location of axon terminals in dorsal spinal cord (lamina II). E, F, Higher magnification of C, D. G, H, The plasma membrane was not permeabilized so that extracellular AMP hydrolytic activity could be assayed. Arrowheads point to neurites emanating from cultured neurons. AMP (6 mm in A, B, G, H and 3 mm in C–F) was used as substrate, and buffer was pH 7.0. Scale bars: (in B, H) A, B, G, H, 50 μm; (in D) C, D, 500 μm; (in F) E, F, 200 μm.