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. Author manuscript; available in PMC: 2010 Feb 23.
Published in final edited form as: Int J Cancer. 2008 Sep 15;123(6):1376–1384. doi: 10.1002/ijc.23667

FIGURE 4.

FIGURE 4

Ectopic expression of PDEF inhibits cell growth, induces apoptosis, downregulates survivin promoter activity and endogenous survivin expression in a highly invasive P11 (2008) ovarian cancer cell line. (a) MTT assay performed for detection of proliferation pcDNA3.1 PDEF P11 cells (PDEF+) and mock pcDNA3.1 P11 cells (PDEF−). (b) Ectopic expression of PDEF (using 4 µg pcDNA3.1 PDEF) efficiently inhibited endogenous survivin expression. The relative expression of PDEF and survivin after normalization to GAPDH internal control are shown. Total mRNA extracts were collected 48 hr posttransfection and analyzed by 1-step RT-PCR (Qiagen). (c) Detection of apoptosis in pcDNA3.1 PDEF P11 cells (PDEF+) and mock pcDNA3.1 P11 by cell death detection ELISA assay. High level of apoptosis was induced when 4 µg of pcDNA3.1 PDEF vector was used for transfection (p = 0.00002). (d) Luciferase assay showing that ectopic expression of PDEF in the PDEF-negative P11 ovarian cancer cell line significantly decreased survivin promoter-driven luciferase activity. Means ± SD derived from 3 independent assays (p = 0.00004).