Table 1. Parameters Used To Model the Dissociation of DCC-SSB from Different Lengths of ssDNA, Induced by wtSSB^a.

	dT70	dT35	poly(dT)
fluorescence enhancementb
initial complex	4.5	4.0	4.3
other intermediates	1.6	2.0	2.6
k+1 (μM−1 s−1)	4.9	6.0	42
k+2 (μM−1 s−1)	3.4	0.61	33
k−2 (s−1)c	0.3	0.3	0.3
k+3 (s−1)c	9.7	9.7	9.7

^aThe models are shown in the schemes in Figure 5, and rate constants are defined such that step n has forward and reverse rate constants k_+n and k_−n, respectively.

^bThis ratio is the fluorescence of DNA-bound DCC-SSB relative to that of free DCC-SSB for either the initial complex, bound in the 70-base mode, or all other intermediates in the schemes, which may be in approximately the 35-base mode. The exception was the assumption that DCC-SSB·wtSSB·dT₃₅ had the same fluorescence as free DCC-SSB.

^cThe values for these two rate constants were optimized for the poly(dT) trace and those values fixed for other DNA lengths.