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. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Biochemistry. 2010 Feb 2;49(4):709–717. doi: 10.1021/bi901495y

FIG. 2. Effect of D639Y and R768W mutations on GC activity of RetGC1.

FIG. 2

A, B. Recombinant WT RetGC1 and its mutants present in HEK293 membrane fraction were assayed for guanylyl cyclase activity in the presence of 1 mM free Mg2+, 10 µM GCAP1 and 2 mM EGTA (A) or 5 mM Mn2+ (B) The activities are standardized for RetGC1 content in each assay, equalized with the wild type as a standard. The average of three independent measurements is shown. For other conditions of the assay see "Experimental Procedures". C. Immunoblotting probed with anti-RetGC1Cat antibody and analyzed by densitometry was used to equalize the RetGC1 content between the HEK293 membrane preparations.