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. Author manuscript; available in PMC: 2011 Feb 2.

Published in final edited form as: Biochemistry. 2010 Feb 2;49(4):709–717. doi: 10.1021/bi901495y

FIG. 4 — A. The cell:nucleus GCAP1-GFP fluorescence intensity ratio among the HEK293 cells that co-express GCAP1-GFP with WT (●), D639Y (□), R768W (Δ) and R838S () RetGC1. The cell:nucleus fluorescence intensity ratio is the ratio between the maximal GFP fluorescence intensity in the whole cell versus its maximal fluorescence intensity inside the nucleus. Each data point represents a single cell. B. Average ratio (mean ± standard error, cell number) for GCAP1-GFP co-expressed with WT (10.6 ± 1.4, n = 25), D639Y (1.2 ± 0.1, n = 26), R768W (1.2 ± 0.04, n = 20) and R838S (9.3 ± 0.9, n = 26) RetGC1; *p<0.01 different from wild type.

Inline graphic — A. The cell:nucleus GCAP1-GFP fluorescence intensity ratio among the HEK293 cells that co-express GCAP1-GFP with WT (●), D639Y (□), R768W (Δ) and R838S () RetGC1. The cell:nucleus fluorescence intensity ratio is the ratio between the maximal GFP fluorescence intensity in the whole cell versus its maximal fluorescence intensity inside the nucleus. Each data point represents a single cell. B. Average ratio (mean ± standard error, cell number) for GCAP1-GFP co-expressed with WT (10.6 ± 1.4, n = 25), D639Y (1.2 ± 0.1, n = 26), R768W (1.2 ± 0.04, n = 20) and R838S (9.3 ± 0.9, n = 26) RetGC1; *p<0.01 different from wild type.