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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunol. 2009 Sep 14;183(7):4569–4582. doi: 10.4049/jimmunol.0901734

Figure 1. Interaction of monoclonal antibodies with KIR3DL1*015 point mutants.

Figure 1

Each 3DL1*015 mutant has a substitution that occurs naturally in another KIR3DL1/S1 allotype. Jurkat cells transiently transfected with each mutant were tested for binding to three monoclonal antibodies: DX9, Z27 and 177407. To correct for differential expression of the mutants, the phycoerythrin (PE) fluorescence due to antibody binding was normalized to the fluorescence due to the green fluorescent protein attached to the cytoplasmic tail of each 3DL1. The binding was then normalized to that obtained for ‘wild type’ 3DL1*015. Shaded black are mutants giving statistically significant differences in binding compared to 3DL1*015. Error bars represent SEM derived from three independent experiments. Statistical significance of differences in binding was calculated using the Student's t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.