Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunol. 2009 Sep 14;183(7):4569–4582. doi: 10.4049/jimmunol.0901734

Figure 3. Mutation at lineage-specific sites and comparison of anti-KIR3DL1 antibodies.

Figure 3

Panel A shows the effects on antibody binding of substitution at the seven sites that distinguish the *005 and *015 KIR3DL1 lineages. In each 3DL1*015 mutant one of the sites has been changed to the residue present in 3DL1*005. Panel B shows the effects on antibody binding of substitution at the six sites that distinguish KIR3DS1 from KIR3DL1. In each 3DL1*015 mutant one of the sites has been changed to the residue present in 3DS1*013. Panel C compares the binding of eight different monoclonal anti-KIR3DL1 antibodies to three natural variants -- 3DL1*004, *005, *015 -- and the 3DL1*015-W283L mutant. 3DL1*004, which is not expressed at cell surfaces, is a negative control (12). The methods of data collection and analysis are as described in the legend to Figure 1.