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A representative protein immunoassay. Two types of pre-encoded microbeads coated with different receptor antibodies (Blicharz et al. 2009) are immobilized in wells etched in silicon. Antigen is then incubated with the array and binds its respective microbead. Next a biotinylated detection antibody is incubated with the array, and finally labeled with a streptavidin conjugated fluorescent reporter.
