Table 2. Summary of identified phosphorylated proteins and phosphorylated sites in porcine lens proteins by IMAC followed by LC-MS/MS analysis.

Protein [Accession number]	Fragment	Phosphopeptides	Designation
αB-crystallin	12–22	RPFFPFHSPSR	Ser-19
[Q7M2W6]	12–22	RPFFPFHSPSR	Ser-21
	57–69	APSWIDTGLSEMR	Ser-59
	57–69	APSWIDTGLSEMR	Thr-63
	73–82	DRFSVNLDVK	Ser-76
	124–149	IPADVDPLTITSSLSSDGVLTVNGPR	Ser-139
	164–174	EEKPAVTAAPK	Thr-170
αA-crystallin	13–21	ALGPFYPSR	Ser-20
[P02475]	55–65	TVLDSGVSEVR	Ser-59
	55–65	TVLDSGVSEVR	Ser-62
	55–70	TVLDSGVSEVRSDRDK	Ser-66
	79–88	HFSPEDLTVK	Ser-81
	79–88	HFSPEDLTVK	Thr-86
	146–157	VPSGVDAGHSER	Ser-148
	146–157	VPSGVDAGHSER	Ser-155
	158–173	AIPVSREEKPSSAPTS	Ser-173
Beta-crystallin B1	8–21	ASATAAVNPGPDGK	Ser-9
[Q007T1]	8–21	ASATAAVNPGPDGK	Ser-11
	88–107	VRSIIVTSGPWVAFEQSNFR	Ser-90
	90–107	SIIVTSGPWVAFEQSNFR	Ser-95
	185–199	VSSGTWVGYQYPGYR	Thr-189
Beta-enolase	81–89	KLSVVDQEK	Ser-83
[Q1KYT0]	257–269	YDLDFKSPDDPSR	Ser-263
Heat shock protein beta-1	13–20	SPSWDPFR	Ser-13
[Q5S1U1]	13–20	SPSWDPFR	Ser-15
Glucose-6-phosphate isomerase	227–241	EWFLQSAKDPSAVAK	Ser-232
[NP_999495]	448–461	ELQAAGKSPEDFEK	Ser-455

In the “Phosphopeptides” column, the phosphorylation sites are underlined.