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. 2010 Feb 16;10:12. doi: 10.1186/1472-6750-10-12

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Copyright ©2010 Zhong et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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IC₅₀of protein microarray immuntodetection and ci-ELISA. Upper panels: Dose-response curves for (A) CL and (B) SM₂from ci-ELISA and protein microarray. The log of the hapten concentration (x-axis) was plotted against the percentage of inhibition (y-axis), which is (OD_sample/OD_control) × 100%. The control group OD was considered to be the point of 100% activity. The IC₅₀was determined by non-linear regression analysis. Lower panels: fluorescent signals from the protein microarray slides for (C) CL and (D) SM₂. The concentration of the standard solution for each slide is: 1: 0 ng/ml; 2: 1 ng/ml; 3: 5 ng/ml; 4: 10 ng/ml; 5: 20 ng/ml; 6: 100 ng/ml; 7: 1000 ng/ml.