Figure 6.
DHA blocks NFκB p65 nuclear translocation. (A) 1 × 107DC were preincubated with (50 μM) DHA or (1 μM) Rosi for 24 h, followed by LPS treatment for 1 h. Presence of NFκB p65 in DC nuclear extracts was determined by binding to κB containing oligonucleotides. Results are expressed as relative activity, i.e. absorbance values above those observed in the absence of LPS stimulation. (B) DC were pretreated with DHA for 24 h, followed by LPS stimulation for 40 min, and cell lysates were subjected to Western blot analysis for IκBα. Numbers represent relative densitometric levels. One experiment out of three is shown.