Table 2.
Specific primers used for real-time quantitative PCR
| Gene name1 | Sequence | Ta | Product size (bp) | Genbank accession no. |
|---|---|---|---|---|
| 18S rRNA | F: 5'-GCGGCTTTGGTGACTCTA-3' R:5'-CTGCCTCCTTGGATGTG-3' |
60°C | 194 [238-431] |
NR_002170.3 |
| ACC | F: 5'-ATG TTT CGG CAG TCC CTG AT-3' R: 5'-TGT GGA CCA GCT GAC CTT GA-3' |
60°C | 133 [4810-4942] |
EF618729 |
| FAS | F: 5'-AGC CTA ACT CCT CGC TGC AAT-3' R: 5'-TCC TTG GAA CCG TCT GTG TTC-3' |
58°C | 196 bp [504-699] |
AY183428 |
| SREBP-1 | F: 5'-GCG ACG GTG CCT CTG GTA GT-3' R: 5'-CGC AAG ACG GCG GAT TTA-3' |
62°C | 218 bp [194-411] |
AF102873 |
| LPL | F: 5'-AAC TTG TGG CTG CCC TAT-3' R: 5'-GAC CCT CTG GTG AAT GTG-3' |
55°C | 367 bp [453-819] |
X62984 |
| HSL | F: 5'-GCT CCC ATC GTC AAG AAT C-3' R: 5'-TAA AGC GAA TGC GGT CC-3' |
55°C | 262 bp [2407-2668] |
AJ000482 |
| PPAR-γ | F: 5'-GAT TTC TCC AGC ATT TCC A-3' R: 5'-GCT CTT CGT GAG GTT TGT T-3' |
60°C | 184 bp [270-453] |
DQ437884 |
| CPT-1 | F: 5'-ATG GTG GGC GAC TAA CT-3' R: 5'-TGC CTG CTG TCT GTG AG-3' |
62°C | 321 bp [681-1001] |
AY181062 |
| A-FABP | F: 5'-CAG GAA AGT CAA GAG CAC CA-3' R: 5'-TCG GGA CAA TAC ATC CAA CA-3' |
55°C | 227 bp [234-460] |
AJ416020 |
1 All porcine gene sequences.