Table 2.
Similarity of cDNA-AFLP sequences to a variety of datasets:
| Fragment ID | Gene annotation | Dataset Comparison | ||||
|---|---|---|---|---|---|---|
| A | B | C | D | E | ||
| BLASTn | BLASTx | BLASTx | BLASTx | BLASTn | ||
| 176.2-G41-48I | putative disease resistance protein [Malus × domestica] | 34 | 25 | 22 | 21 | 28 |
| 176.1-G41-48I | unknown [Malus × domestica] | 34 | 24 | 23 | 23 | 30 |
| 171-G41-48I | Probable WRKY transcription factor 53 (WRKY DNA-binding protein 53) | 36 | 24 | 18 | 24 | 30 |
| 54.2-M.26R | DNA topoisomerase II [Malus × domestica] | 36 | 24 | 20 | 24 | 26 |
| 175-G41-48I | putative WRKY transcription factor 30 [Vitis aestivalis] | 38 | 26 | 23 | 24 | 32 |
| 131.4_G41_48_OE | hypothetical protein pNG7269 [Haloarcula marismortui ATCC 43049] gb|AAV44969 | 38 | 20 | 20 | 23 | 28 |
| 37-G41-48R | 40 | 20 | 18 | 22 | 28 | |
| 136.2-G41-2I | hypothetical protein 12.t00009 [Asparagus officinalis] | 40 | 24 | 21 | 23 | 26 |
| 64.4-G41-48OE | Fusarium resistance protein I2C-5-like [Oryza sativa (japonica cultivar-group)] | 42 | 24 | 21 | 22 | 28 |
| 201.3-G41-48I | putative leucine-rich repeat transmembrane protein kinase [Malus × domestica] | 44 | 41 | 39 | 21 | 30 |
| 200.1-G41-48I | Probable WRKY transcription factor 29 | 52 | 64 | 22 | 54 | 26 |
| 213-G41-48I | Probable WRKY transcription factor 65 (WRKY DNA-binding protein 65) | 56 | 68 | 22 | 52 | 26 |
| 221-G41-48I | Probable WRKY transcription factor 65 (WRKY DNA-binding protein 65) | 56 | 66 | 22 | 51 | 30 |
| 7.2_M.26_2 | hypothetical protein RT0201 [Rickettsia typhi str. Wilmington] gb|AAU03684.1| cons | 64 | 21 | 21 | 22 | 62 |
| 190-G41-48I | Leucine-rich repeat [Medicago truncatula] | 418 | 21 | 22 | 22 | 28 |
| 175.2_G41_48I | beclin 1 protein [Malus × domestica] | 541 | 22 | 23 | 30 | 30 |
| 81_G41_48I | AT5 g56010/MDA7_5 [Arabidopsis thaliana] | 841 | 22 | 23 | 30 | 769 |
| 176.3_G41_48I | protein kinase [Malus × domestica] | 280 | 22 | 25 | 30 | 26 |
| 171.1_G41_48I | protein kinase [Malus × domestica] | 107 | 23 | 24 | 35 | 28 |
| 4.3_M.26_2I | MYB11 [Malus × domestica] | 628 | 24 | 21 | 24 | 646 |
| 165_M.26_2R | protein kinase [Malus × domestica] | 168 | 24 | 44 | 51 | 28 |
| 201_M.26R | LYTB-like protein [Malus × domestica] | 692 | 24 | 24 | 24 | 26 |
| 98_G41_48 | putative chalcone isomerase 4 [Glycine max] | 1195 | 24 | 22 | 26 | 805 |
| 3.3_M.26_2I | Os08 g0162600 [Oryza sativa (japonica cultivar-group)] | 289 | 24 | 22 | 20 | 289 |
| 137.1_G41_48_I | dbj|BAC57824.1| unknown | 472 | 25 | 25 | 27 | 24 |
| 115_G41_2I | chalcone synthase [Malus × domestica] | 714 | 26 | 24 | 22 | 26 |
| 200_G41_48I | soluble NSF attachment protein [Malus × domestica] | 496 | 26 | 25 | 26 | 28 |
| 4.2_M.26_2I | ATP binding/kinase/protein serine/threonine kinase [Arabidopsis thaliana | 936 | 29 | 26 | 29 | 936 |
| 142_G41_48I | flag-tagged protein kinase domain of putative mitogen-activated protein kinase kinase | 654 | 37 | 22 | 34 | 293 |
| 166_M.26_2R | protein kinase [Malus × domestica | 414 | 44 | 49 | 20 | 26 |
| 1.2_M.26_2I | putative hydroquinone glucosyltransferase; arbutin synthase [Malus × domestica] | 444 | 62 | 23 | 60 | 32 |
| 112_G4148I | aquaporin 2 [Bruguiera gymnorhiza] | 793 | 116 | 166 | 21 | 34 |
| 201_G41_48I | translation initiation factor eIF-4A [Malus × domestica] | 309 | 121 | 24 | 21 | 30 |
| 137.2_G41_48I | hypothetical protein [Citrus × paradisi] | 507 | 141 | 23 | 21 | 498 |
| 205_G41_48I | glyceraldehyde-3-phosphate dehydrogenase [Panax ginseng] | 765 | 176 | 22 | 24 | 414 |
ESTs expressed preferentially under fire blight challenge (A), A. thaliana compatibility ESTs (B); A. thaliana incompatibility ESTs (C), similar to A. thaliana Salicylic Acid Response ESTs (D), and Malus EST in tissue challenged by E. amylovora found by Norelli et al, (2009) by suppression subtractive hybridization (SSH) (E). Gene annotations were determined by most informative BLASTx comparision below a predetermined threshold of 1e-3. NA indicates BLASTn similarity score below (A) or above (B-E).