Table 1.
Genes, primers and amplicon characteristics
| Gene name | Molecular function | Accesion | Tblastx e-value | Primer sequences (forward/reverse) | Length (bp) | Efficiency |
| ACT | Actin 11 | SGN-U208507 (At3 g12110.1) | 2e-110 | TGCACTCCCACATGCTATCCT/TCAGCCGAAGTGGTGAAAGAG | 114 | 1.75 ± 0.07 |
| CYP | Cyclophilin | SGN-U207595 (At2 g21130.1) | 1.9e-75 | AGGCTCATCATTCCACCGTGT/TCATCTGCGAACTTAGCACCG | 111 | 1.64 ± 0.10 |
| EF1α | Elongation factor 1-alpha | SGN-U207468 (At5 g60390.1) | 0 | CCTGGTCAAATTGGAAACGG/CAGATCGCCTGTCAATCTTGG | 103 | 1.62 ± 0.08 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | SGN-U209515 (At1 g42970.1) | 9.2e-79 | AACAACTCACTCCTACACCGG/GGTAGCACTAGAGACACAGCCTT | 135 | 1.83 ± 0.09 |
| RPS13 | Ribosomal protein S13 | SGN-U208260 (At4 g00100.1) | 4e-77 | CAGGCAGGTTAAGGCAAAGC/CTAGCAAGGTACAGAAACGGC | 114 | 1.70 ± 0.04 |
| RAN1 | GTP-binding nuclear protein | SGN-U207968 (At5 g20010.1) | 1e-119 | AAGCTCCCACCTGTCTGGAAA/AACAGATTGCCGGAAGCCA | 103 | 1.71 ± 0.07 |
| SAND | SAND family protein | SGN-U210443 (At2 g28390.1) | 8.2e-76 | CTTACGACGAGTTCAGATGCC/TAAGTCCTCAACACGCATGC | 135 | 1.61 ± 0.12 |
| TUB | Tubulin beta-6 chain | SGN-U207876 (At5 g12250.1) | 6e-147 | TGGAAACTCAACCTCCATCCA/TTTCGTCCATTCCTTCACCTG | 114 | 1.61 ± 0.05 |
| UBQ | Polyubiquitin | SGN-U207515 (At4 g02890.2) | 8e-107 | TGGAGGATGGAAGGACTTTGG/CAGGACGACAACAAGCAACAG | 153 | 1.67 ± 0.02 |
Selected candidate reference genes accessions are shown as identifiers of Solanaceae Genomics Network (SGN) and Arabidopsis TAIR databases (in brackets). Homologous Arabidopsis genes were determined on the basis of tblastx e-values