Table 2.
Optimal genes for quantification of individual and mixed organs in each Petunia line.
| Mitchell | ||||||||
| Statistic | Flower | Leaf | ||||||
| A | B | C | D | A+B+C+D | A | C | A+C | |
| M geNorm |
EF1α (0.05) RPS13 (0.05) |
SAND (0.14) UBQ (0.14) |
EF1α (0.13) RPS13 (0.13) |
RAN1 (0.08) RPS13 (0.08) |
RAN1 (0.47) SAND (0.47) |
EF1α (0.11) RPS13 (0.11) |
RAN1 (0.14) SAND (0.14) |
EF1α (0.37) RPS13 (0.37) |
| M qBasePlus |
ACT (0.55) RPS13 (0.56) |
EF1α (0.60) RPS13 (0.60) |
CYP (0.60) SAND (0.64) |
EF1α (0.30) RAN1 (0.34) |
EF1α (0.80) SAND (0.82) |
RPS13 (0.77) EF1α (0.78) |
SAND (0.93) RAN1 (0.93) |
EF1α (0.85) RAN1 (0.92) |
| CV qBasePlus |
ACT (0.05) SAND (0.15) |
RPS13 (0.07) CYP (0.25) |
CYP (0.09) SAND (0.11) |
EF1α (0.05) TUB (0.14) |
EF1α (0.25) SAND (0.28) |
RPS13 (0.14) RAN1 (0.18) |
SAND (0.12) RAN1 (0.18) |
RAN1 (0.20) EF1α (0.21) |
| Min. number | 2 (0.04) | 2 (0.07) | 2 (0.12) | 2 (0.05) | 4 (0.15) | 2 (0.10) | 2 (0.13) | 2 (0.12) |
| V30 | ||||||||
| Statistic | Flower | Leaf | ||||||
| A | B | C | D | A+B+C+D | A | C | A+C | |
| M geNorm |
RAN1 (0.11) UBQ (0.11) |
TUB (0.12) CYP (0.12) |
RPS13 (0.23) UBQ (0.23) |
ACT (0.02) CYP (0.02) |
RAN1 (0.45) ACT (0.45) |
TUB (0.07) RAN1 (0.07) |
RPS13 (0.09) TUB (0.09) |
RAN1 (0.20) UBQ (0.20) |
| M qBasePlus |
CYP (0.30) RAN1 (0.33) |
CYP (0.66) TUB (0.69) |
SAND (0.63) CYP (0.63) |
RPS13 (0.29) EF1α (0.30) |
ACT (2.27) RAN1 (2.44) |
TUB (0.34) UBQ (0.36) |
SAND (0.27) RPS13 (0.29) |
UBQ (0.49) RPS13 (0.51) |
| CV qBasePlus |
CYP (0.05) TUB (0.10) |
RAN1 (0.09) CYP (0.11) |
SAND (0.10) ACT (0.25) |
EF1α (0.06) CYP (0.06) |
ACT (0.70) RAN1 (0.82) |
UBQ (0.06) TUB (0.09) |
SAND (0.03) RPS13 (0.06) |
UBQ (0.14) RPS13 (0.16) |
| Min. number | 2 (0.10) | 2 (0.07) | 2 (0.09) | 2 (0.04) | NA | 2 (0.04) | 2 (0.03) | 2 (0.07) |
M values computed by geNorm and qBasePlus allow to rank optimal reference genes. For each organ and mix of organs the two top-ranked genes are shown. The number of genes required for a reliable quantification is established using a Pairwise Variation (PV) cut-off of 0.15; n is the the minimum number of control genes required NA means that no one pairwise variation was under the proposed cut-off.