Table 3.
Rank position | NormFinder | BestKeeper | qBasePlus M | qBasePlus CV | geNorm M | Consensus | ||||||
Mitchell | V30 | Mitchell | V30 | Mitchell | V30 | Mitchell | V30 | Mitchell | V30 | Mitchell | V30 | |
1 | EF1α | UBQ | CYP | CYP | EF1α | ACT | EF1α | RAN1 | RAN1 | RPS13 | EF1alpha | CYP |
2 | CYP | RAN1 | EF1α | EF1α | SAND | RAN1 | SAND | CYP | SAND | UBQ | SAND | RAN1 |
3 | RPS13 | ACT | RPS13 | ACT | RAN1 | CYP | RPS13 | ACT | UBQ | RAN1 | RPS13 | ACT |
4 | UBQ | GAPDH | ACT | SAND | RPS13 | TUB | RAN1 | TUB | EF1alpha | CYP | RAN1 | UBQ |
5 | ACT | RPS13 | UBQ | UBQ | CYP | RPS13 | CYP | RPS13 | RPS13 | ACT | CYP | RPS13 |
6 | SAND | SAND | TUB | GAPDH | UBQ | UBQ | UBQ | UBQ | CYP | TUB | UBQ | TUB |
7 | TUB | EF1alpha | SAND | RPS13 | TUB | EF1alpha | TUB | EF1alpha | TUB | EF1alpha | TUB | EF1alpha |
8 | GAPDH | TUB | RAN1 | RAN1 | ACT | SAND | ACT | GAPDH | ACT | SAND | ACT | SAND |
9 | RAN1 | CYP | GAPDH | TUB | GAPDH | GAPDH | GAPDH | SAND | GAPDH | GAPDH | GAPDH | GAPDH |
Gene expression data were analyzed using five statistical parameters in both Petunia lines. Each column refers to a gene suitability ranking computed by one statistical tool, taking into account all data of a Petunia line.