Table 3.
Comparison of gamma gliadins encoded by Butte 86 contigs to proteins in NCBI and proteins encoded by contigs from other EST assemblies.
| Butte 86 Contig # | # Amino acids | NCBI Accession #1 | Identity | TaGI 10.0 Contig # | Identity | TaGI 11.0 Contig # | Identity | US Wheat Genome Project Contig # | Identity2 | HarvEST 1.14 Contig # | Identity2 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 328 | [GenBank: AAK84774.1] | 326/337 | TC2347103 | 194/197 | TC2918573 | 181/193 | 185244 | nd | 14880 | 328/328 |
| TC250086 | 328/328 | TC280138 | 327/328 | 19014,5 | nd | ||||||
| TC250043 | 326/337 | ||||||||||
| 2 | 327 | [GenBank: AAK84779.1] | 326/327 | TC250310 | 327/327 | TC327998 | 327/327 | 188714 | nd | 4446 | 327/327 |
| TC3330203 | 160/198 | 149393,4 | nd | ||||||||
| 3 | 302 | [GenBank: AAA34272.1] | 300/302 | TC249992 | 300/302 | TC308951 | 299/302 | 18764 | 299/302 | 2079 | 302/302 |
| 4 | 302 | [GenBank: ACJ03466.1] | 302/302 | TC250000 | 302/302 | TC308951 | 276/302 | 18752 | 301/302 | 149393,4 | nd |
| TC337354 | 269/302 | 149453 | 212/230 | ||||||||
| 5 | 358 | [GenBank: ACJ03521.1] | 342/362 | TC233870 | 357/358 | TC3573196 | 357/358 | 188737 | nd | 15356 | 358/358 |
| TC250310 | 306/358 | ||||||||||
| 6 | 285 | [GenBank: ABO37962] | 283/285 | TC249991 | 285/285 | TC3299763 | 254/264 | 18814 | 285/285 | 15101 | 285/285 |
| TC332920 | 265/285 | ||||||||||
| 7 | 291 | [GenBank: ACJ03428.1] | 290/291 | TC232926 | 291/291 | TC296897 | 290/291 | 18753 | 289/291 | 4448 | 290/291 |
| 19008 | 291/291 | ||||||||||
| 8 | 332 | [GenBank: AAK84774.1] | 317/337 | TC250043 | 317/337 | TC280138 | 313/332 | 185244 | nd | 14880 | 314/332 |
| TC2347103 | 194/197 | TC281237 | 318/339 | 2713 | 176/176 | 46049 | nd | ||||
| TC250086 | 314/332 | TC2918573 | 183/185 | 19014,5 | nd | ||||||
| 474323 | 188/188 | ||||||||||
| 93 | 289 | [GenBank: ACJ03517.1] | 266/294 | TC2499963 | 204/227 | TC293526 | 283/289 | 16926 | 288/289 | 14942 | 288/289 |
| TC2500313 | 251/255 | ||||||||||
| 10 | 286 | [GenBank: AAA34272.1] | 284/302 | TC249992 | 284/302 | TC308951 | 283/302 | 18764 | 283/302 | 2079 | 286/302 |
| TC3377603 | 142/180 | 188424 | nd | 2095 | 283/286 | ||||||
| 113 | 163 | [GenBank: ACJ03465.1] | 163/163 | TC233421 | 163/163 | TC277980 | 163/163 | 165853 | 163/163 | 4328 | 163/163 |
1 accession with highest score in BLASTp search of non-redundant protein sequences using BLOSUM62, no compositional adjustments, no filters, no masks. Database last searched on 5/08/09.
2 protein identities that were not determined because consensus sequences of contigs contained frameshifts or stop codons in the reading frame are indicated by nd.
3 partial coding sequence.
4 consensus sequence contains frameshift or stop codon in reading frame.
5 complement of consensus sequence.
6 contig contains a portion of a LMW-GS sequence at 5' end.
7 encodes alpha gliadin.
8 encodes identical protein to Butte 86 contig, but does not contain any ESTs from Butte 86.
9 encodes LMW-glutenin subunit.