Table 5.
Butte 86 gamma gliadins identified by MS/MS from wheat flour.
| Butte 86 contig # | Band # 1 | Total # peptides 2 | % Coverage3 | Unique peptides4 | Enzyme used to generate peptide5 |
|---|---|---|---|---|---|
| 2 | 126 | 14 | 36.0 | SQQQQVGQGSL | CH |
| QQLPQPQQPQQSFPQQQR | CH | ||||
| SQQQQVGQGSLVQGQGIIQPQQPAQL | CH | ||||
| NIQVDPSGQVQWLQQQLVPQLQQPL | CH | ||||
| LQQPQQPFPQPQQQLPQPQQPQQ | TH | ||||
| 4 | 188 | 22 | 56.5 | SIIMQQEQRQGVQIRRPL | CH |
| LQPQQPQQSFPQQQQPL | CH | ||||
| VSPDCSTINAPF | CH | ||||
| VSPDCSTINAPFASIVVGIGGQ | CH | ||||
| LQPQQPQQSFPQQQQPLIQL | CH | ||||
| LQPQQPQQSFPQQQQPLIQLSL | CH | ||||
| CQQPQQTIPQPHQTF | CH | ||||
| IIMQQEQRQG | TH | ||||
| IIMQQEQRQGVQ | TH | ||||
| LQPQQPQQSFPQQQQPLIQ | TH | ||||
| VDPGYQVHWPQQQPFPQPQQP | TH | ||||
| VHWPQQQPFPQPQQP | TH | ||||
| NFLLQQCNPVSLVSSLISMILPR | TR | ||||
| 5 | 145 | 31 | 44.8 | VPPECSIIRAPF6 | CH |
| IQPSLQQR | CH | ||||
| IQPSLQQRL | CH | ||||
| SQQQQLGQGTL6 | CH | ||||
| QSFPQQQRPF | CH | ||||
| ILLPLSQQQQL6 | CH | ||||
| TQQPQQPFPQFQQPHQPF6 | CH | ||||
| VQGQGIIQPQQLAQLEAIRSL6 | CH | ||||
| SQQQQLGQGTLVQGQGIIQPQQL6 | CH | ||||
| SQQQQLGQGTLVQGQGIIQPQQLAQLEAIRSL6 | CH | ||||
| ILLPLSQQQQLGQGTL6 | CH | ||||
| SQQQQLGQGTLVQGQGIIQPQQLAQL6 | CH | ||||
| SQQPQQAFPQPQQTFPHQPQQQVPQPQQPQQPF | CH | ||||
| HQPQQQFPQPQQPQQSFPQQQRPF | CH | ||||
| HQPQQQFPQPQQPQQSFPQ | CH | ||||
| AFPQPQQTFPHQPQQQVPQPQQPQQPF | TH | ||||
| FHQPQQQFPQPQQPQQ | TH | ||||
| FHQPQQQFPQPQQPQQSFPQQQRP | TH | ||||
| IQPSLQQR | TR | ||||
| QLAQLEAIR | TR | ||||
| PFIQPSLQQR | TR | ||||
| QSFPQQQRPFIQPSLQQR | TR | ||||
| 6 | 186 | 16 | 36.5 | RQPQQPF | CH |
| ASIVAGISGQ | CH | ||||
| YQQPQQTFPQPQ | CH | ||||
| LAQIPRQ | TH | ||||
| IQILRPLFQ | TH | ||||
| IIQPQQPAQYEVIRS | TH | ||||
| FRQPQQPFY | TH | ||||
| IIQPQQPAQYE | TH | ||||
| YQQPQQTFPQPQQ | TH | ||||
| FRQPQQPFYQQPQQTFPQPQQ | TH | ||||
| FYQQPQQTFPQPQQ | TH | ||||
| 7 | 170 | 13 | 43.4 | LQPHQPF | CH |
| ASIVASIGGQ | CH | ||||
| QGVQILVPL | CH | ||||
| SQQQQVGQGIL | CH | ||||
| SQQQQVGQGILVQGQGIIQPQQPAQL | CH | ||||
| VYVPPYCST | TH | ||||
| LQPHQPFSQQPQQ | TH | ||||
| APFASIVASIGGQ | TR | ||||
| QPFPQQPQQPYPQQPQQPFPQTQQPQQPFPQSK | TR | ||||
| 11 | 149 | 4 | 27.6 | YQQQQVGQGTLVQGQGIIQPQQPAQL | CH |
| SFPQQQPPF | TH | ||||
| 17 | 180 | 7 | 24.9 | ASIVADIGGQ | CH |
| 87 | 22.0 | KAPFASIVADIGGQ | CH | ||
| APFASIVADIGGQ | TR | ||||
| 38 | 169 | 16 | 37.5 | ANIDAGIGGQ | CH |
| 108 | 39.7 | GIIQPQQPAQLEGIRSLVL | CH | ||
| VPPNCSTINVPY | CH | ||||
| VPPNCSTINVPYANIDAGIGGQ6 | CH | ||||
| VTILRPLFQ6 | TH | ||||
| INVPYANIDAGIGGQ | TH | ||||
| NFLLQQCNHVSLVSSLVSIILPR | TR | ||||
1 detailed in Vensel et al. (in preparation).
2 obtained by interrogation of "subset" database with MS/MS data.
3 excluding 19 amino acid signal peptide.
4 sequences of peptides obtained by MS/MS that are not shared by other Butte 86 gamma gliadins. Peptide containing the extra cysteine is indicated in italics.
5 CH, chymotrypsin; TH, thermolysin; TR, trypsin.
6 peptides that distinguish Butte 86 sequence from closest NCBI match.
7 MS/MS data consistent with either Butte 86 gamma gliadin #1 or #8.
8 MS/MS data consistent with either Butte 86 gamma gliadin #3 or #10.