Table 1.

Typical DIGE images as analysed by DeCyder Image Analysis Software. (Figure 7a,c shows the images of proteins labelled by Cy3. Figure 7b,d shows the images of proteins labelled by Cy5. Peak volumes of each spot were compared against each other; 2.0-fold difference in peak volume was used as a cut-off to determine the difference in protein expression between test and control. In all DIGE gels there were areas showing results of up- and downregulation. These spots were used for protein identification.)

electrophoretic area	name	function summary	expression resulting from static culture
1	procollagen-proline, 2-oxoglutarate 4-dioxygenase and prolyl 4-hydroxylase	collagen formation	2.50±1.13
2	vimentin	cytoskeleton (intermediate filament)	2.93±0.51
3	actin	cytoskeleton (microfilament)	3.55±1.57
4	beta-galactoside-binding lectin precursor (galectin precursor)	regulation of cell adhesion	3.63±0.82
5	Hsp70 and GRP78 (BiP)	chaperone	−2.39±0.29
6	GRASP55	reassembling of Golgi apparatus	−2.81±0.82
7	RNase inhibitor	RNA degradation inhibition	−2.77±0.77
8	zyxin	focal adhesion forming	−2.83±0.89
9	caldesmon	calmodulin and actin-binding protein	−5.03±0.99
10	alpha-enolase	enzyme in glycolytic pathway	−2.58±0.31
11	cathepsin D	lysosomal protease	−2.23±0.31
12	heat shock protein 27 (Hsp27)	chaperone	−4.23±0.39