Figure 8.

Axin-mediated downregulation of TCF-4 is p53-independent. BE1 cells were transfected with wild-type p53 or β-catenin siRNA, respectively, cotransfection of wild-type p53 and AxinΔβ-ca was also performed in BE1 cells. A549 cells were transfected with p53 siRNA or β-catenin siRNA, respectively, and cotransfection of β-catenin siRNA and AxinΔβ-ca, p53 siRNA and AxinΔβ-ca were also performed in A549 cells. (A&E) Forty-eight hours after transfection total RNA and RT-PCR analysis was performed using the indicated primers. EGFP and control siRNA served as control groups. (B&F) Quantification of mRNA expression after transfection. (C&G) Western blot analysis was performed 48 h after transfection using the indicated antibodies. (D&H) Quantification of protein expression after transfection * P < 0.05, ** P < 0.01.