Skip to main content
. Author manuscript; available in PMC: 2010 Feb 23.
Published in final edited form as: J Immunol. 2008 Jan 15;180(2):1207–1216. doi: 10.4049/jimmunol.180.2.1207

FIGURE 5.

FIGURE 5

Effect of H. pylori viability and H. pylori-cell interaction, OipA, and cag PAI on IL-18 mRNA expression. H. pylori (wild-type H. pylori, heat-killed wild-type H. pylori strains, oipA mutants, or cag PAI mutants) (MOI of 100) were added to exponentially growing cells in 6-well plates. We inhibited the interaction of wild-type H. pylori strains (MOI of 100) with cells using a polyester membrane to inhibit any direct bacteria-cell interaction. After 3-h coculture, IL-18 protein levels released from human cells were determined by absolute quantitative real-time RT-PCR. The expression levels of IL-18 mRNA were expressed as the ratio of IL-18 mRNA to GAPDH mRNA. At least three independent cocultures were performed and each was measured in triplicate. Data are expressed as mean ± SE. *, p < 0.001 compared with uninfected control and #, p < 0.001 compared with wild-type H. pylori infected samples by nonpaired t test.