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. 2010 Feb 24;5(2):e9387. doi: 10.1371/journal.pone.0009387

Figure 1. In vitro fertilization rates with cryopreserved C57BL/6J sperm in cell culture inserts and wells.

Figure 1

The bar patterns, in groups of 4, indicate the volume of cryoprotectant used to extend the sperm prior to freezing. From L to R: 105 µL, 180 µL, 400 µL and 800 µL. Numbers within each group of 4 bars indicate IVF Protocol: Protocol 1, 6.5 mm insert; Protocol 2, 6.5 mm well; Protocol 3, 12 mm insert; Protocol 4, 12 mm well. Data are presented as mean ± SEM, n = 4. Significance of differences is described in text.