Table 4. Performance characteristics of Pentaplex PCR system with reference to “normal DNA” for the identification of MMR-deficient CRCs.

No. of Markers Displaying Allelic Variation	The Five Marker Panel*				The Three Marker Panel**
	Sensitivity (%)	Specificity (%)	PPV (%)#	NPV (%)+	Sensitivity (%)	Specificity (%)	PPV (%)#	NPV (%)+
5	76.3 (67.7–83.2)	100 (96.3–100)	100 (95.8–100)	78.6 (70.6–84.8)
4	87.7 (80.4–92.5)	100 (96.3–100)	100 (96.3–100)	87.6 (80.3–92.5)
3	93.9 (87.9–97.0)	100 (96.3–100)	100 (96.5–100)	93.4 (87.0–96.8)	88.6 (87.5–93.2)	100 (96.3–100)	100 (96.3–100)	88.4 (81.1–93.1)
2	95.6 (90.1–98.1)	100 (96.3–100)	100 (96.6–100)	95.2 (89.2–97.9)	93.9 (87.9–97.0)	100 (96.3–100)	100 (96.5–100)	93.4 (87.0–96.8)
1	97.4 (92.5–99.1)	84.9 (76.5–90.6)	88.1 (81.3–92.7)	96.6 (90.3–98.8)	97.4 (92.5–99.1)	92.9 (86.1–96.5)	94.1 (88.3–97.1)	96.8 (91.1–98.9)

Results are expressed as percentages (%), with 95% confidence intervals in parentheses.

*The Five Pentaplex Marker Panel composed of BAT25, BAT26, NR21, NR24 and NR27 markers.

**The Three Pentaplex Marker Panel composed of BAT26, NR21 and NR27 markers.

^#PPV  =  positive predictive value.

⁺NPV  =  negative predictive value.