Figure 6. CHEB42A increases current from the mammalian DEG/ENaC protein ASIC1a.

A–C. Effect of CHEB42A on H⁺-gated ASIC1a currents. Xenopus oocytes were injected with ASIC1a, GFP or CheB42a cDNAs alone or in combinations indicated. Bathing solution pH was reduced to 5 during time indicated. A. example of H⁺-gated currents from the ASIC1a channel. B. CHEB42A increases ASIC1a H⁺-gated currents. Asterisk indicates P<0.01 (two-tail t-test). Number of oocytes studied: 9 GFP, 9 CheB42a and GFP, 20 ASIC1a and GFP, and 27 ASIC1a and CheB42a. Data are mean ± SEM. C. CHEB42A did not affect the pH dependence of ASIC1a current. Closed symbols indicate ASIC1a and CHEB42A (n = 15) and open symbols indicate ASICla + GFP (n = 12). D. CHEB42A did not affect ASIC1a surface expression. Experiment was done as in Fig. 5B.