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. 2010 Feb 24;5(2):e9408. doi: 10.1371/journal.pone.0009408

Figure 2. PEX11 is an integral membrane protein that forms a high molecular weight complex.

Figure 2

A. Duplicate 100 microgramme mixed light membrane fractions (10,000 g pellet) from Arabidopsis suspension cells was subjected to extraction by 0.1 M sodium carbonate. Soluble and insoluble fractions were separated by SDS-PAGE (with or without DTT) and immunoblotted with PEX11 affinity purified antibody. B. 100 microgrammes of protein from a peroxisome enriched organelle fraction was solubilised in the indicated concentration of detergent and the solubilised proteins were separated on a 3–8% native gel and immnoblotted with anti-PEX11 affinity purified antiserum.