Figure 5. Disruption of the laminar architecture in the MAM-treated rat brain.

A–D: Tuj1 expression, a marker for differentiated neurons, normally shows a tightly organized pattern in the E19 control brain. In brains from MAM-treated animals Tuj1 staining is spread throughout the entire neocortex and there was no obvious intermediate zone or organized cortical plate structure. E–H: Chondroitan Sulfate Proteoglycan (CSPG) staining was present in the pial basement membrane and the subplate region of the E19 control rat (E and arrow in G). In the brains from MAM-treated animals the subplate was disrupted and not visible as an organized structure (H). Scale bars: A, B, and E = 200mm; C, D = 50mm.