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. 2010 Feb 15;137(4):641–650. doi: 10.1242/dev.041913

Fig. 6.

Fig. 6.

crb limits Dl-dependent N transactivation and S2 cleavage. (A,B) crb11A22 mutant clones marked by lack of GFP (green) as outlined by the white dotted line in the follicular epithelium (FE) of the egg chamber. At stage 5, no Hnt (red) expression in either the crb11A22 clones or WT tissue is detected. tsg101 clones (B) marked by lack of GFP (green) lead to ectopic Hnt expression (red) at stage 5. (C) Number of Dl- and NICD-positive vesicles in WT and crb11A22 eye discs. WT, n=6; crb, n=8; t-test: P>0.05. (D) Number of Dl- and NECD-positive vesicles in WT and crb11A22 eye discs. WT, n=5; crb, n=4; ***, t-test: P<0.001. (E) The effect of S2 cleavage inhibition upon the number of dextran vesicles positive for NECD and Dl in WT and crb11A22 mutant discs. Left-hand bars, control with no S2 cleavage inhibitor; right-hand bars, 5 mM OP. WT, n=6; crb, n=6; ***, t-test: P<0.001. n, number of discs. Vesicles were counted in multiple sample areas per disc. Error bars represent the s.e. Scale bars: 25 μm.