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. Author manuscript; available in PMC: 2011 Mar 5.
Published in final edited form as: FEBS Lett. 2010 Jan 30;584(5):1006–1010. doi: 10.1016/j.febslet.2010.01.049

Fig. 3. PTTG1 is downregulated with KLF6 overexpression.

Fig. 3

HepG2 cells were transfected with KLF6 and RNA and protein collected after 24hrs. (A) QRTPCR on cDNA showed a 12-fold over-expression of KLF6 with a 24% decease of PTTG1 (P < 0.0005). (B) 60ug of protein from total cell extracts were loaded on 10% SDS-PAGE gel and submitted to western blot analysis for KLF6 and PTTG1. GAPDH served as the protein loading control. There was approximately 50% decrease in PTTG1 protein with several fold overexpression in KLF6. The data presented in (B) is representative of at least 3 independent experiments.