Fig. 7. Silencing of PTTG1 abrogates siKLF6 induced cell proliferation.

HepG2 cells were transfected with either siKLF6, siPTTG1, siControl, or a combination there of for 24, 48, and 72 hours. Cells were incubated with 1 μCi/ml [3H]thymidine for 2 hours before collection, and analyzed for radioactive thymidine incorporation. The 24hr time point is set to baseline. siPTTG1 shows a decrease in proliferation compared with siControl at both 48hr and 72hr (P < 0.05), whereas siKLF6 increases proliferation at both time points. Co-transfection of siPTTG1 and siKLF6 abrogat