Skip to main content
. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Nat Neurosci. 2010 Feb 14;13(3):319–326. doi: 10.1038/nn.2491

Figure 7.

Figure 7

Neutrophils infiltrate into the corpus callosum at early stages of cuprizone-induced demyelination. Cells from corpus callosum of Cxcr2+/+ mice fed with cuprizone for 3 days, 5 days, 7 days and 9 days and 6 weeks were stained with Ly6GFITC, F4/80PE, CD45PerCP and Gr1APC antibodies. In comparison with control, the CD45int/Ly6G+ neutrophils increased significantly at day 7 (left column). Analysis for F4/80 (macrophage marker) demonstrated that the Ly6G+ population is uniformly negative (middle column). The myeloid marker Gr1 (Ly6C) is correlated with the neutrophil marker Ly6G (right column). Each group has two mice. These data represent three separate experiments.