Figure 1.
Cationic perfluorocarbon nanoparticles. Liquid perfluorocarbon based nanoparticles are formulated with the cationic lipid 1,2-Dioleoyl-3-Trimethylammonium-Proane (DOTAP) and the fluorescent lipid 1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine-N-(Carboxyfluorescein) within the surrounding phospholipid-surfactant monolayer (illustrated in the cartoon and TEM image). Electrostatic interactions between the charged lipids of the nanoparticles and the negatively charged phosphate backbone of siRNA lead to the formation of transfection complexes.
