Fig. 1.

Oncopeltus fasciatus giant. (A) Alignment of the binding domain of the co-repressor, CtBP, from the Oncopeltus, Drosophila, Tribolium and Nasonia orthologs of Giant. (B) The conserved leucine zipper domain of Giant from the four insects. (C) Cartoon diagram showing approximate locations of the CtBP binding and leucine zipper domains of Oncopeltus Giant. Boxed region is the predicted open reading frame, predicted to encode a 210 amino acid protein. Single lines show approximate relative lengths of the 5′ (100 bp) and 3′ (800 bp) untranslated regions. Lines below the cartoon diagram show approximate lengths and positions of the fragments used to synthesize in situ probes and double-stranded RNA. Fragment A was used for probe synthesis, fragment B to synthesize both probe and double-stranded RNA, fragment C was used only to synthesize double-stranded RNA, and fragment D, located in the 3′ untranslated region, was also only used to synthesize double-stranded RNA. Figure not to scale.