Table 1.
Mean confidence limits of analytical method of fluconazole in plasma.
| Parameter | Unit | Confidence Limits |
|---|---|---|
| Linearity (triplicate) | μg mL−1 | 0.4 – 100.0 |
| Linear regression (n=3 curves) | r2 = 0.998 | |
| LOD (0.2 μg mL−1) (n=10) | CV% | 2.6 |
| LOQ (0.4 μg mL−1) (n=10) | CV% | 3.2 |
| Absolute recovery (n=24) | % | 98.6 |
| Relative recovery (n=24) | % | 104.2 |
| Precision/within-day (n=9) | CV% | |
| 90.0 μg mL−1 | 1.44 | |
| 50.0 μg mL−1 | 4.60 | |
| 1.2 μg mL−1 | 3.21 | |
| Precision/between-days (n=18) | CV% | |
| 90.0 μg mL−1 | 0.99 | |
| 50.0 μg mL−1 | 1.51 | |
| 1.2 μg mL−1 | 0.74 | |
| Accuracy/within-day (n=9) | % (mean+/− SD) | |
| 90.0 μg mL−1 | 98.1 – 100.1 | |
| 50.0 μg mL−1 | 92.6 – 95.8 | |
| 1.2 μg mL−1 | 101.3 – 107.5 | |
| Accuracy/between-days (n=18) | % (mean+/− SD) | |
| 90.0 μg mL−1 | 97.6 – 100.7 | |
| 50.0 μg mL−1 | 89.3 – 99.1 | |
| 1.2 μg mL−1 | 103.7 – 105.1 | |
| Stability/thawing cycles | SE% | |
| Cycle 1(n=12) | 0.06 | |
| Cycle 2(n=12) | 1.65 | |
| Cycle 3(n=12) | 1.22 |
Abbreviations: coefficient of variation (CV%), standard deviation of mean (SD), systematic error (SE%), higher and lower limits (mean+/− SD) for accuracy and precision. Symbol: r2: coefficient of determination.