Fig. 1.

IL-1 increases the expression of C/EBPδ mRNA and protein. (A) Hep3B cells were either left untreated or incubated with IL-1 (100 U/ml) (− and +, respectively) for the indicated period of time and total cellular RNA was subjected to RT-PCR using primers for C/EBPδ or GAPDH. The amplification products were size-fractionated by agarose gel electrophoresis along with molecular size markers (M). −RT shows the outcome of a reaction in which the reverse transcriptase step was omitted (RNA from untreated cells at 0 h was used) and NC indicates a reaction that contained water instead of cDNA. (B) Western blot analysis was performed on extracts from cells that were either left untreated (0 h) or incubated with IL-1 (100 U/ml) for the indicated period of time, and antiserum against C/EBPδ or β-actin. The results shown in both panels A and B are representative of three independent experiments.