Fig. 4.

Subcellular localization of Mamu-B molecules can be assigned to the α1 and α2 domains. a Scheme of chimeric constructs. The chimeric constructs of Mamu-I*010201 and Mamu-B*06002 are named according to the exchanged domains (α1, α2 or α1 and α2 domains together). SP signal peptide, TM/CT transmembrane region and cytoplasmic tail, AcGFP Aequorea coerulescens green fluorescent protein. b K562 cells were transiently transfected and analysed with W6/32 monoclonal antibody and secondary goat anti-mouse Pe-Cy5-coupled antibody 2 days post-transfection by flow cytometry. Lower panel shows confocal laser microscopy (×63 magnification) merged images of AcGFP-tagged Mamu class I molecules (green) and W6/32 (red). Numbering allows for rapid identification of the respective constructs shown in (a). All experiments were performed at least three times with similar results and a representative experiment is shown