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. 2009 Nov 30;42(2):105–115. doi: 10.3858/emm.2010.42.2.012

Figure 5.

Figure 5

Western blotting was performed to detect ERK1/2 expression levels in the brain of the phenylketonuria mouse model (BTBR-Pahenu2). ERK1/2 protein expression in (A) the cerebrum, (B) the cerebellum, and (C) the brain stem was demonstrated and quantified using a bioimaging analyzer. Whole cell lysates of PC-12 cells treated with (D) 0.9 mM phenylalanine were used for detection of phosphorylated ERK1/2 and total ERK1/2. Western blotting was performed to detect transthyretin expression levels in PC-12 cells treated with (E) 0.9 mM phenylalanine after preincubation with ERK inhibitor II. The cells were exposed to phenylalanine for 7 days. Transthyretin levels were normalized to GAPDH levels. Values are expressed as the mean ± SD (n = 3-4). *P < 0.05, **P < 0.01 vs wild-type mice or DMSO- and/or PBS-treated controls using Student's t-test.