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. 2010 Jan 7;42(2):116–121. doi: 10.3858/emm.2010.42.2.013

Figure 3.

Figure 3

Genotyping of transfectant viruses by multiplex RT-PCR. PCR products of X-31ca reassortants, rgXca-H9N2 (between X-31ca and A/Chicken/Kores/MS96/96) and rgXca-H5N1 (between X-31ca and A/Indonesia/2005) were fractionated by 1% agarose gel and stained with ethidium bromide. Lane 1 and 4: specific for X-31caPB2 (1,002 bp): NP (853 bp): PB1 (447 bp); Lane2 and 5: specific for X-31ca-PA (730 bp): NS (521 bp): M (326 bp); Lane3: specific for haemagglutinin (981 bp) and neuraminidase (552 bp) of H9N2; Lane6: specific for haemagglutinin (510 bp) and neuraminidase (1,286 bp) of H5N1.