Figure 2.

GFW suppresses TNBS-induced TNF-α expression in rat colon. The colon tissues were either counterstained with hematoxylin and eosin (left column) or immunostained with TNF-α antibody (middle and right columns). A, B, and C, untreated control; D, E, and F, treated with TNBS; G, H, and I, treated with TNBS and 5-ASA; J, K, and L, treated with TNBS and GFW. The TNF-α -immunoreactive cells were presented as dark blue color. MM, muscularis mucosa; SL, submucosa layer; ML, muscle layer; TA, tunica adventitia. Scale bars = 160 µm.