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. 2010 Jan 7;42(2):143–154. doi: 10.3858/emm.2010.42.2.016

Figure 6.

Figure 6

The effects of GFW on TNF-α-induced ROS generation in HT-29 cells (A) and DPPH-generated free radical (B). In the experiment of (A), the cells were treated with GFW for 1 h prior to TNF-α (10 ng/ml) stimulation. After 3 h, cytochrome c (80 µM) was added to each well and incubated for 15 min at 37℃. The level of cytochrome c reduction represents cellular ROS production. The absorbance was read at 550 nm by a spectrophotometer. Data are expressed as the mean ± SEM of three independent experiments. In the experiment of (B), GFW was incubated with DPPH for 30 min, the absorbance at 517 nm due to DPPH radical was determined. DPPH radical scavenging activity was calculated from the following equation in which H and Ho were optical density of solvent with and without sample, respectively. Radical scavenging activity (%) = {(Ho - H)/Ho} × 100. The data represent the mean ± SEM. *P < 0.01 compared to untreated control group. #P < 0.01 compared to TNF-α-treated group.