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. 2010 Feb 1;120(3):756–767. doi: 10.1172/JCI36066

Figure 1. Dgat1 mRNA levels are increased in both adipocytes and macrophages from aP2-Dgat1 mice.

Figure 1

(A) Increased Dgat1 mRNA levels in whole WAT and in adipocyte (Adip) and SVF fractions of aP2-Dgat1 mice fed a chow diet. mRNA levels were measured by qPCR and normalized for each gene product to levels in whole WAT for WT (n = 5–6 per group; *P < 0.05). Samples were pooled for adipocytes and SVF. (B) Increased Dgat1 mRNA levels in P-Mϕ of aP2-Dgat1 mice fed a chow or high-fat diet. mRNA levels were measured by qPCR (n = 4–8 per group; *P < 0.05). Mean Ct values for Dgat1, Dgat2, and Emr1 (F4/80) were 27.6, 30.2, and 25.0, respectively, for WT chow-fed samples and 25.0, 24.0, and 24.9, respectively, for WT high-fat samples. Data for each gene were normalized to WT chow. (C) Increased Dgat1 mRNA levels in BMD-Mϕ of aP2-Dgat1 fed a chow diet. mRNA levels were measured by qPCR (n = 4–8 per group; *P < 0.05). (D) Representative immunoblot demonstrating increased DGAT1 protein in BMD-Mϕ of aP2-Dgat1 mice.