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. 2010 Feb 1;120(3):756–767. doi: 10.1172/JCI36066

Figure 7. DGAT1 expression levels correlate with neutral lipid storage in BMD-Mϕ.

Figure 7

(A) WT macrophages take up oleate and palmitate similarly but incorporate more oleate into cellular TG. WT BMD-Mϕ (n = 3–4 per group) were incubated with 100 μM of either radiolabeled oleate or palmitate for 24 hours, and dpm were measured from total cellular lipid and the TG fractions after isolation by TLC (*P < 0.05). (B) BMD-Mϕ from aP2-Dgat1 mice (n = 3–4 per group) exhibit more prominent lipid droplets after 24 hours incubation with 200 μM oleate. Scale bars: 25 μm. (C) Neutral lipid content (measured by Nile Red fluorescence) of Dgat1–/–, WT, and aP2-Dgat1 BMD-Mϕ after 24 hours in medium alone or with 200 μM oleate or 150 μM palmitate (n = 3–4 per group; *P < 0.05 vs WT control, **P < 0.05 versus WT for same treatment condition).

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