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. 2010 Feb 15;120(3):744–755. doi: 10.1172/JCI39678

Figure 4. WFS1 forms a complex with the proteasome and ATF6α.

Figure 4

(A) WFS1 was subjected to IP from INS1 832/13 cells using an anti-WFS1 specific antibody. IPs were subjected to IB with anti–alpha 5 20S proteasome and anti-WFS1 antibodies. (B) IB analysis measuring CREB, actin, and PDI levels using whole cell lysates or ER-isolated lysates of INS1 832/13 cells. ER-isolated lysates of INS1 832/13 cells were also subjected to fractionation using a 10%–40% glycerol gradient. Fractions were analyzed by IB using anti–alpha 5 20s proteosome, anti-ATF6α, and anti-WFS1 antibodies. Lanes were run on separate gels and were not contiguous. (C) WFS1 was subjected to IP from a mixture of fractions 10 and 11 using an anti-WFS1 antibody, and IP products were subjected to IB analysis using anti-alpha 5 20s proteosome, anti-ATF6α, and anti-WFS1 antibodies. ATF6 was subjected to IP from a mixture of fractions 9 and 12, and IP products were analyzed by IB with anti–alpha 5 20s proteosome and anti-ATF6α (n = 3).