Figure 4. Rab25 deficiency promotes intestinal adenoma formation in Apc^Min/+ mice.

(A) Gross appearance of small intestine from Apc^Min/+;Rab25^+/+ (+/+) and Apc^Min/+;Rab25^–/– (–/–) mice. Rab25-deficient mice showed an increase in tumor numbers and size throughout the intestine. (B) Anatomical distribution of polyps in small intestine (SI) of Apc^Min/+;Rab25^+/+ (+/+) and Apc^Min/+;Rab25^–/– (–/–) mice. Polyp number was determined in the proximal, middle, and distal intestine as well as in the total intestines of Apc^Min/+;Rab25^+/+ (+/+) (n = 7), Apc^Min/+;Rab25^+/– (+/–) (n = 16), and Apc^Min/+;Rab25^–/– (–/–) (n = 12) mice. The distal intestine showed a greater increase in the number of polyps than the proximal intestine. Apc^Min/+;Rab25^+/– mice also showed a significant increase in tumor numbers that was intermediate between wild-type and Rab25-deficient mice. Data represent mean ± SEM. (C) Representative Swiss rolls of proximal and distal intestine from Apc^Min/+;Rab25^+/+ (+/+) and Apc^Min/+;Rab25^–/– (–/–) mice. Both proximal and distal intestine from Apc^Min/+;Rab25^–/– (–/–) mice showed an increase in tumor number. Scale bars: 1 mm.