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. 2010 Feb 15;120(3):850–858. doi: 10.1172/JCI41013

Figure 2. Disruption of NT-3 autocrine loop triggers NB cell death.

Figure 2

(A) NT-3 immunostaining on the CLB-Ge2 cell line 24 hours after transfection with scrambled siRNA (siRNA scr) or with NT-3 siRNA (siRNA NT-3). Insets depict control without primary antibody. Original magnification, ×32. (B and C) Cell death induction in CLB-Ge2, CLB-VolMo, SHEP-CLB, and IMR32 cell lines was quantified after transfection with either scrambled siRNA or a mix of 3 siRNAs targeting NT-3, using relative caspase-3 activity assay (B) or Toxilight assay (C). (D and E) Cell death induction in CLB-Ge2, CLB-VolMo, or IMR32 cell lines was quantified in cells treated with anti-TrkC blocking antibody (α TrkC) or without (control) anti-TrkC antibody, using relative caspase-3 activity assay (D) or TUNEL assay (E). For the TUNEL assay, a representative labeling of TUNEL-positive cells is shown (top panel, control cells; bottom panel, cells treated with anti-TrkC blocking antibody). Original magnification, ×20. (F) Effect of anti-TrkC blocking antibody on stage 4 NB. Tumoral cells were directly dissociated from the surgical biopsy and were plated for 24 hours in presence (+) or in absence (-) of treatment. (B–F) Data represent mean ± SEM. *P < 0.05, 2-sided Mann-Whitney test, compared with control.