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. 2009 Dec 15;159(3):543–553. doi: 10.1111/j.1476-5381.2009.00533.x

Figure 2.

Figure 2

Detection of SK1 and effect of DMS on SK1 activity. (A) RNA was extracted from PVSMC and subjected to RT-PCR with SK1 gene-specific primers. A single amplicon of the expected size (480 bp) was obtained. Lane 1 – size markers; lane 2 – RT-PCR of SK1 amplicon. (B) DMS was confirmed to inhibit recombinant SK1 activity in vitro (expressed as pmol·min·µg−1 protein) in a concentration-dependent manner. Recombinant SK1 activity was assayed in triplicate using cell lysates derived from HEK 293 cells over-expressing hSK1.